76 PNL Volume 19 1987 RESEARCH REPORTS
CONFIRMING DATA FOR MAPPING ISOZYMIC LOCUS Aat-p
Swiecicki, W. K. Plant Breeding Station, Wiatrowo, Poland
and B. Wolko
An isozyme linkage map for Pisum was published by Weeden in
1985 (1), providing an additional group of markers useful for gene
mapping, as well as for genotype/cultivar characterization (2).
One of these enzymes was analyzed at Wiatrowo, i.e. Aspartate
aminotransferase - Aat-p (=Glutamate oxaloacetate transaminase
Got-1). The testerline WL 851 (allele B2, slow for Got-1) was
crossed with Wt 3838, cv. 'Arabal' (allele B1, fast). These
lines also have different alleles of genes A and Lf. Plants of
the F1 generation showed the dominant phenotype for A and Lf as
well as a heterozygotic pattern for Aat-p (Got-1). An undisturbed
monohybrid segregation in F2 was observed for A, Lf , as well as
for Aat-p (Table 1A). A 3:1 segregation for Lf-lf was obtained on
the basis of considering individuals with the first flowering node
below 6 as recessives. For Aat-p (Got-1) co-dominant type of
inheritance was observed (1:2:1) but for linkage calculations
allele B1 of Got-1 (fast variant) was added to the heterozygotes
to get the 3:1 segregation pattern. The dihybrid segregation be-
tween Aat-p (Got-1) with A and Lf produced the following CrO values (Table 1B).
Aat-p---------------------27.0-------------A-------18.7--------Lf
I-----------------------------32.0-----------------------------I
The above confirms Weeden's data showing linkage between
Aat-p and A as 30 units and also extends the linkage relations to
the Lf locus of chromosome 1.
1. Weeden, N.F. 1985. The Pea Crop, P.D. Hebblethwaite,
M.C. Heath, and T.C.K. Dawkins, eds. Butterworths,
London. pp. 55-66.
2. Wolko, B. and W.K. Swiecicki. 1987. PNL 19:89.

 

 

Table 1. Phenotypes distribution in an F2 population segregating for Aat-p (Got-1) from the cross Wt 851 x Wt 3838.

 

A. Monohybnd F1 segregation

Aat-p

150

aat-p
45

Total
195

Chi-square

(3 1)

0.38

A
148

a

46

194

0.17

Lf
150

lf
43

173

0.002

B. Joint segregation of Aat-p with A and Lf

A, Aat-p

105

A, aat-p

42

a, Aat-p

43

a, aat-p

3

Total

194

Joint
chi -square

10.09**

Recomb.
fract.

27.01

S.E.
6.6

Aat-p, Lf
94

Aat-p, lf

39

aat-p, Lf
36

aat-p, lf
4

173

6.25*

32.02

6.7

A, Lf

115

A, lf

15

a, Lf
14

a, lf

28

172

49.16**

18.68

3.4

 

 

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