PNL Volume 16 1984
PRODUCTION OF 2N POLLEN AND LINKAGE RELATIONS OF CALYX CARPELLAR1S
Myers, J. R. and E. T. Gritton Department of Agronomy
University of Wisconsin, Madison, WI USA
A line designated X1415 and segregating for a floral mutant was
provided to us by L. G. Cruger, Del Monte Corp., San Leandro, CA USA.
Tests of allelism demonstrated that this mutant was allelic to calyx
carpellaris (cc) segregating in the G.10B line studied by Gottschalk (1)
(supplied by S. Blixt, Weibullsholm Plant Breeding Institute,
Landskrona, Sweden). The mutant in the X1415 line is male and female
fertile, but highly self-sterile. The G.10B allele exhibits greatly
reduced male and female fertility. However, mutant F2 plants from
crosses between G.10B and marker lines showed male and female fertility
similar to X1415.
The cc mutant produced two distinct classes of pollen. One class
was normal haploid (n) pollen while the other class consisted of
diploid (2n) pollen. Production of 2n pollen was affected by environ-
ment and genetic background. The X1415 mutant allele produced moderate
amounts of 2n pollen while no 2n pollen was observed in mutant plants of
the G.10B line. Some F2 cc plants of both X1415 and G.10B exhibited
high 2n pollen production when crossed to WL-1514. However, no plant
was observed to produce exclusively 2n pollen.
The mechanism responsible for 2n pollen appears to be parallel
spindles (rather than normal perpendicular spindles) during the second
meiotic division (2). With this type of meiotic aberration, one expects
not only dyads from parallel spindles, but triads (two n microspores and
one 2n microspore) from intermediate spindle orientation. Both dyads
and triads were observed in cc anthers.
Crosses were attempted between the autotetraploid pea line
Falensky-4 (A-, Le-, R-, I-, Cc-) and cc plants of X1415 (carrying a,
le, r, i, cc), using cc as the male. One cross was successful and three
seeds were obtained. Progeny from these seeds were observed from the F1
to the F5 generation and while F1 resembled the female parent, the F2
and subsequent generations segregated for a, le , r, i, and cc.
Tetraploidy in the progeny was verified by chromosome counts and reduced
Gottschalk (1) demonstrated that cc was conditioned by a pair of
homozygous recessive alleles at a locus. Our data agree on the in-
heritance and we obtained linkage with r, tl, and coch on chromosome 7.
Crossover values between r and cc were not homogeneous, precluding
calculation of a pooled crossover value for these two genes. Overall,
the data suggest the following gene order: r-5-tl-7-cc-27-coch.
1. Gottschalk, W. 1964. Bot. Studien 14:1-359.
2. Mok, D. W. S. and S. J. Peloquin. 1975. Can. J. Genet. Cytol.